Molecular profile of patients with myelofibrosis: a 10-year experience.

OBJECTIVE: To analyze the karyotype test and myeloid panel with next-generation sequencing findings in patients with myelofibrosis, and to compare transplant characteristics in patients referred for bone marrow transplantation. METHODS: Retrospective, single-center study with patients diagnosed with myelofibrosis treated at Hospital Israelita Albert Einstein between 2010 and 2020. RESULTS: A total of 104 patients with myelofibrosis were examined. Patients who had not been submitted to tests in our service were excluded. The final sample comprised 69 patients. Of these 69, 56 were submitted to karyotyping and 22 to myeloid panel with next-generation sequencing. Karyotype was normal in 60% of the patients and altered in 40%. The prevalence of high-risk molecular mutations was higher in patients referred for bone marrow transplantation (100% versus 50%). The median follow-up of transplant patients was 2.4 years and the overall survival at 2 years was 80% (95%CI: 62-100%). CONCLUSION: The molecular analysis enables estimating the patient's risk and thus instituting more aggressive treatment such as bone marrow transplant for patients at higher risk, being a relevant tool to guide therapy. Given the significance of molecular analysis for therapeutic decision-making in myelofibrosis, collection and disclosure of data on the prevalence of cytogenetic changes and findings of next-generation sequencing in affected patients is important.

Molecular profile of patients with myelofibrosis: a 10-year experience

ABSTRACT Objective To analyze the karyotype test and myeloid panel with next-generation sequencing findings in patients with myelofibrosis, and to compare transplant characteristics in patients referred for bone marrow transplantation. Methods Retrospective, single-center study with patients diagnosed with myelofibrosis treated at Hospital Israelita Albert Einstein between 2010 and 2020. Results A total of 104 patients with myelofibrosis were examined. Patients who had not been submitted to tests in our service were excluded. The final sample comprised 69 patients. Of these 69, 56 were submitted to karyotyping and 22 to myeloid panel with next-generation sequencing. Karyotype was normal in 60% of the patients and altered in 40%. The prevalence of high-risk molecular mutations was higher in patients referred for bone marrow transplantation (100% versus 50%). The median follow-up of transplant patients was 2.4 years and the overall survival at 2 years was 80% (95%CI: 62-100%). Conclusion The molecular analysis enables estimating the patient's risk and thus instituting more aggressive treatment such as bone marrow transplant for patients at higher risk, being a relevant tool to guide therapy. Given the significance of molecular analysis for therapeutic decision-making in myelofibrosis, collection and disclosure of data on the prevalence of cytogenetic changes and findings of next-generation sequencing in affected patients is important.

Neuroimaging and motor development of twins with congenital microcephaly associated with Zika virus: a case report / Neuroimagem e desenvolvimento motor de gêmeas com microcefalia congênita por Zika virus: estudo de caso

Objective: We aimed to describe a case of congenital microcephaly caused by Zika virus infection in a monozygotic twin pregnancy. Methods: Transfontanelle ultrasonography and cranial computed tomography revealed different lesion patterns for both twins with congenital microcephaly caused by Zika virus infection. Motor development assessments were performed using the Alberta Infant Motor Scale and the Gross and Motor Function Measure before, during, and after physiotherapy. Results: The evaluations showed differences in motor acquisition between the twins. The values in the first case were much lower than those in the second case, which showed more motor delay. Conclussion: The present study showed that despite the twins being monozygotic, the effects of neurological lesions as revealed by neuroimaging were worse in the first case, and even with two weekly rehabilitation sessions, motor development over time was considerably worse in the twin in case 1 than in the other twin.

Objetivo: Descrever um caso de microcefalia congênita causada pela infecção do Zika vírus em uma gestação gemelar monozigótica. Métodos: A ultrassonografia transfontanelar e a tomografia computadorizada de crânio revelaram diferentes padrões de lesão para ambos gêmeos com microcefalia congênita causada pela infecção do Zika vírus. As avaliações do desenvolvimento motor foram realizadas por meio da Escala Motora Infantil de Alberta (EMIA) e da Medida de Função Motora Grossa antes, durante e após o tratamento fisioterapêutico. Resultados: As avaliações mostraram diferenças na aquisição motora entre os gêmeos. Os valores nas avaliações do primeiro caso foram bem menores do que os do segundo caso, que apresentou maior atraso motor. Conclusão: O presente estudo mostrou que apesar dos gêmeos serem monozigóticos, os efeitos das lesões neurológicas reveladas por exames de neuroimagem foram piores no primeiro caso, e mesmo com duas sessões semanais de reabilitação, o desenvolvimento motor ao longo do tempo foi consideravelmente pior no gêmeo do caso 1 quando comparado ao caso 2.

Weathering Resistance of Waterborne Polyurethane Coatings Reinforced with Silica from Rice Husk Ash.

Waterborne polyurethanes (WPUs) are interesting materials for coatings when compared to solvent-based polyurethanes, once that reducing the concentration of volatile organic compounds that are harmful for human health and the environment. However, the WPU has low weathering resistance. In order to improve this behavior among others properties, inorganic fillers has been added in these systems. SiO2 particles from various sources, mainly, from agro-industrial waste, as rice husk has attracted the scientific and technological interest. In this study, the accelerated weathering essay was performed in waterborne polyurethane (WPU)/ silica (from rice husk ash) composites in order to evaluate the thermal and physical changes in these materials. These composites were prepared by two distinct methods: in situ polymerization and blending method. The highest resistance to thermal degradation and to accelerated weathering was reached with WPU/silica composites obtained by blending method due the interactions between SiO2 particles and the polymer matrices. Blending method for preparation WPU/silica composites proved to be a simpler and faster method, with no drawback for large scale application.

Uso de equipamentos de proteção individual por técnicos de enfermagem / Use of individual protection equipment by nursing technicians

Objetivo: verificar a utilização dos Equipamentos de Proteção Individual pelos Técnicos de Enfermagem de uma Unidade Hospitalar num município do interior de Pernambuco. Método: estudo descritivo, exploratório, de abordagem quanti-qualitativa, realizado na Unidade Hospitalar do município de Carnaíba-PE com 20 técnicos de enfermagem. A coleta de dados foi realizada por meio da aplicação de um questionário semi-estruturado contendo 09 questões. Os dados foram analisados por média, proporção e números absolutos no programa Microsoft Excel 2007. Resultados: foram entrevistados 20 técnicos de enfermagem e todos alegaram conhecimento dos equipamentos de proteção individual. A luva foi referida como o equipamento de proteção individual mas utilizado e o óculos como o de menor uso, já a máscara foi citada como a que mais causa incômodo. Conclusão: os profissionais entrevistados fazem uso dos equipamentos de proteção individual porque estão cientes do ambiente insalubre no qual laboram.

Objective: to verify the use of Personal Protective Equipment by the Nursing Technicians of a Hospital Unit in a municipality in the interior of Pernambuco. Method: a descriptive, exploratory, quantitative-qualitative study carried out at the Hospital Unit of Carnaíba-PE with 20 nursing technicians. Data collection was performed through the application of a semi-structured questionnaire containing 09 questions. Data were analyzed by mean, proportion and absolute numbers in the Microsoft Excel 2007 program. Results: 20 nursing technicians were interviewed and all reported knowledge of personal protective equipment. The glove was referred to as the personal protective equipment but used and the glasses as the least use, and the mask was cited as the most uncomfortable. Conclusion: the professionals interviewed use personal protective equipment because they are aware of the unhealthy environment in which they work.

Avaliação das radiografias periapicais: estudo realizado na clínica de graduação da Faculdade de Odontologia da UFMG / Evaluation of periapical radiographs taken by dental students in the dental school of Universidade Federal de Minas Gerais, Brazil

Este estudo visou constatar e quantificar quais são os erros encontrados em radiografias periapicais realizadas por alunos de graduação na Clínica de Cirurgia da FOUFMG. Para isso, foram utilizadas 1396 radiografias periapicais, cujo processamento foi manual pela técnica visual em caixas de processamentos portáteis. Os erros foram classificados em sub revelação, sub exposição, super revelação, super exposição, velamento, risco durante o processamento, impressão digital, lavagem inadequada, nível dos líquidos de processamento baixos, angulação vertical, angulação horizontal, meia-lua, enquadramento dos dentes, deformação do filme na manutenção, encartonamento do filme inadequado, risco após o processamento